Interesting facts
Interesting Facts About 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic Acid
2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid, commonly abbreviated as HEPES, is one of the most widely used buffering agents in biological and biochemical research. Here are some notable points about this compound:
- Buffering Capacity: HEPES is particularly valued for maintaining physiological pH levels in biological systems, typically around pH 7.4. This is crucial for experiments involving enzymes and cells that are sensitive to pH changes.
- Biocompatibility: It exhibits excellent biocompatibility and minimal toxicity, making it a preferred choice for cell culture applications.
- Versatility: HEPES is utilized in various applications, including protein purification, microbial culture, and in the preparation of reagents for various biochemical assays.
- Cold Conditions: Unlike many other buffering agents, HEPES maintains its buffering capacity even at lower temperatures, which is beneficial for studies that require refrigeration or cold conditions.
- pKa Value: The pKa of HEPES is around 7.5, which aligns perfectly with the physiological pH range, making it an ideal buffer for biological systems.
Given its many attributes, HEPES has become a staple in numerous laboratories across the world. As noted by many researchers, "HEPES is one of those unsung heroes of biochemistry that contributes significantly to the success of experiments." Whether used in cell culture or molecular biology, its role in maintaining stable reactions cannot be overstated.
In conclusion, 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid is more than just a chemical compound; it is a vital tool that facilitates groundbreaking research in the life sciences.
Synonyms
HEPES
7365-45-9
4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid
1-Piperazineethanesulfonic acid, 4-(2-hydroxyethyl)-
4-(2-Hydroxyethyl)-1-piperazineethane sulfonic acid
hydroxyethylpiperazine ethane sulfonic acid
RWW266YE9I
N-2-Hydroxyethylpiperazine-N'-ethanesulfonate
NSC 166663
N-2-Hydroxyethylpiperazine-N'-ethanesulfonic acid
N-2-Hydroxyethylpiperazine-N-ethane sulfonic acid
N-2-Hydroxyethylpiperazine N',2'-ethanesulfonic acid
EINECS 230-907-9
HEPES [MI]
NSC-166663
BRN 0883043
4-(2-Hydroxyethyl)piperazin-1-ylethanesulphonic acid
EC 230-907-9
5-23-02-00379 (Beilstein Handbook Reference)
NSC166663
4-(2-Hydroxyethyl)-1-piperazineethane sulphonic acid
HYDROXYETHYLPIPERAZINE ETHANE SULFONIC ACID [II]
N-2-HYDROXYETHYLPIPERAZINE N'-2'-ETHANESULPHONIC ACID
HYDROXYETHYLPIPERAZINE ETHANE SULFONIC ACID (II)
HEPES solution
2-(4-(2-hydroxyethyl)piperazin-1-ium-1-yl)ethanesulfonate
2-[4-(2-hydroxyethyl)piperazin-1-ium-1-yl]ethanesulfonate
N 2 Hydroxyethylpiperazine N' 2' ethanesulfonic Acid
N-2-Hydroxyethylpiperazine-N'-2'-ethanesulfonic Acid
CHEBI:46756
N2HydroxyethylpiperazineN'ethanesulfonate
N2HydroxyethylpiperazineN'ethanesulfonic acid
N2HydroxyethylpiperazineNethane sulfonic acid
4(2Hydroxyethyl)1piperazineethanesulfonic acid
4(2Hydroxyethyl)1piperazineethane sulfonic acid
1Piperazineethanesulfonic acid, 4(2hydroxyethyl)
4(2Hydroxyethyl)piperazin1ylethanesulphonic acid
N(2hydroxyethyl)piperazineN'2ethanesulfonic acid
N2Hydroxyethylpiperazine N',2'ethanesulfonic acid
Hydroxyethyl Piperazine Ethanesulfonic Acid Solution
HYDROXYETHYLPIPERAZINE ETHANE SULFONIC ACID [INCI]
2-(4-(2-Hydroxyethyl)piperazin-1-yl)ethanesulfonic acid
2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid
4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID
4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid
MFCD00006158
CHEBI:42334
N-(2-Hydroxyethyl)piperazine-N'-(2-ethanesulfonic acid)
TVZ 7;WAS 13;NSC 166663
2-[4-(2-Hydroxyethyl)-1-piperazinyl]ethanesulfonic acid
2-(4-(2-Hydroxyethyl)piperazin-1-yl)ethane-1-sulfonic acid
2-[4-(2-hydroxyethyl)piperazin-1-yl]ethane-1-sulfonic acid
UNII-RWW266YE9I
HeBES
4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, HEPES, N-(2-Hydroxyethyl)piperazine-N'-(2-ethanesulfonic acid)
Prestwick_256
HEPES, dry powder
HEPES buffered saline
1d1p
1dg9
HEPES, biochemical grade
15(R)-hydroperoxy-EPE
hepes, 99.5%
Epitope ID:190604
N-2-Hydroxyethylpiperazine-N'-2-ethanesulfonic acid
2-(4-(2-Hydroxyethyl)piperazin-1-yl)ethanesulfonicacid
CHEMBL1232545
DTXSID5040382
2-[4-(2-hydroxyethyl)-1-piperazinyl] ethanesulfonic acid
BCP06984
HEPES, >=99.5% (titration)
HY-D0857
HEPES, >=99.0%
s6831
STK033062
AKOS001696205
FH31182
HEPES, >=99.0% (T)
HEPES-buffered saline, pH 6.5 (5X)
BP-31246
BS-17486
DA-64103
DS-015636
HEPES, Vetec(TM) reagent grade, 99.5%
6,11-Dibromo-1,4-dioxaspiro[4.7]undecane
CS-0015167
H0396
NS00004939
EN300-100251
F20297
4-(2-hydroxyethyl)-1-piperazine ethanesulfonate
4-(2-Hydroxyethyl)-1-piperazineethane sulfonate
4-(2-hydroxyethyl)-1-piperazine ethanesulphonate
4-(2-Hydroxyethyl)-1-piperazineethane sulphonate
N-2-Hydroxyethylpiperazine-N-2-ethanesulfonic acid
Q418359
(4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid
4-(2-hydroxylethyl)piperazine-1-ethanesulfonic acid
N-2 hydroxyethylpiperazine-N-2-ethane-sulfonic acid
N-2-Hydroxyethylpiperazino-N'-2-ethanesulfonic acid
4-(2-hydroxyethyl)-1-piperazine ethanesulphonic acid
HEPES-buffered saline, pH 7.0 (2X for transfection)
n-(2-hydroxyethyl)-piperazine-n'-ethanesulfonic acid
4-(2-Hydroxyethyl)piperazine-1-(2-ethanesulfonic acid)
F0001-1858
HEPES, BioUltra, for molecular biology, >=99.5% (T)
Hepes,4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid
Z1335657480
2-[4-(2-hydroxyethyl)-1-piperazinyl]ethane sulfonic acid
2-[4-(2-Hydroxyethyl)-1-piperazinyl]ethanesulfonic acid #
4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid (GMP)
hepes (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid)
HEPES, anhydrous, free-flowing, Redi-Dri(TM), >=99.5%
2-(4-(2-hydroxyethyl)piperazinyl)ethanesulfonic acid (HEPES)
HEPES, BioPerformance Certified, >=99.5% (titration), cell culture tested
HEPES, BioXtra, pH 5.0-6.5 (1 M in H2O), >=99.5% (titration)
HEPES, Pharmaceutical Secondary Standard; Certified Reference Material
HEPES, BioXtra, suitable for mouse embryo cell culture, >=99.5% (titration)
Solubility of 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid
The compound 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid, commonly referred to as HEPES, exhibits notable solubility characteristics that make it particularly useful in biochemical applications. Here are some key points regarding its solubility:
In summary, the solubility properties of 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid are typically **favorable** for its intended applications in biological and biochemical research. As a buffering agent, it holds an essential role in maintaining the desired pH levels in various experimental settings.