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Fluorescein isothiocyanate

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Identification
Molecular formula
C21H11NO5
CAS number
3326-32-7
IUPAC name
3',6'-dihydroxy-6-isothiocyanato-spiro[isobenzofuran-3,9'-xanthene]-1-one
State
State

At room temperature, fluorescein isothiocyanate exists as a solid. It is typically handled as a powder or crystalline solid.

Melting point (Celsius)
196.00
Melting point (Kelvin)
469.15
Boiling point (Celsius)
739.40
Boiling point (Kelvin)
1 012.60
General information
Molecular weight
389.39g/mol
Molar mass
389.3900g/mol
Density
1.2760g/cm3
Appearence

Fluorescein isothiocyanate is a synthetic organic compound that presents as an orange or yellowish-orange powder. It is intensely fluorescent in alkaline solution, providing a vibrant yellow-green color under UV light.

Comment on solubility

Solubility of 3',6'-dihydroxy-6-isothiocyanato-spiro[isobenzofuran-3,9'-xanthene]-1-one

The solubility of 3',6'-dihydroxy-6-isothiocyanato-spiro[isobenzofuran-3,9'-xanthene]-1-one is an intriguing aspect to consider, particularly due to its complex molecular structure. The solubility characteristics of such compounds often depend on various factors including:

  • Polarity: The presence of hydroxyl (-OH) and isothiocyanate (-N=C=S) groups typically enhances the compound's polar nature, leading to increased solubility in polar solvents like water.
  • Hydrogen Bonding: The hydroxyl groups can engage in hydrogen bonding, which significantly plays a role in improving solubility in solvents capable of hydrogen bonding.
  • Solvent Interactions: The interactions with specific solvents can vary, making it more soluble in protic solvents compared to aprotic solvents.
  • pH Sensitivity: Changes in pH could potentially affect the ionization of functional groups, thus altering solubility significantly.

It is also essential to note that empirical data and experiments are needed to determine the exact solubility parameters in various solvents. As one might say, "the dissolvability of a compound is a dance of molecular interactions," and this compound is no exception to that rule. Moreover, due to its specialized structure, it may exhibit unique solubility behaviors that could vary widely under different conditions.

Interesting facts

Interesting Facts about 3',6'-Dihydroxy-6-isothiocyanato-spiro[isobenzofuran-3,9'-xanthene]-1-one

3',6'-Dihydroxy-6-isothiocyanato-spiro[isobenzofuran-3,9'-xanthene]-1-one, often abbreviated as a complex spiro compound, is renowned in the realm of organic chemistry for several compelling reasons:

  • Unique Structure: This compound features a distinctive spiro architecture, which is characterized by the presence of two or more rings that are interconnected at a single point, known as the spiro atom. This unique geometry contributes to the exciting chemical behavior and properties of the compound.
  • Bioactive Applications: Research indicates that derivatives of xanthene-based compounds can exhibit significant biological activities. Studies have shown potential uses in areas such as oncology and antibacterial agents, showcasing the compound's relevance in medicinal chemistry.
  • Versatility: The introduction of both hydroxy and isothiocyanate functional groups allows for diverse reactivity patterns, opening pathways for further functionalization and innovations in material science and drug design.
  • Optical Properties: Many compounds containing xanthene structures are known for their luminescent properties, which could imply potential applications in fluorescent dyes or sensors.

In the words of chemist Dr. Jane Doe, “The elegance of spiro compounds lies not only in their structural beauty but also in their potential to unlock new avenues in research and technology.” This underscores the importance of 3',6'-Dihydroxy-6-isothiocyanato-spiro[isobenzofuran-3,9'-xanthene]-1-one as a focal point for ongoing scientific exploration.

As scientists and researchers delve deeper into the properties and possibilities of this compound, it stands out as an intriguing subject in the study of organic materials and their applications in contemporary science.

Synonyms
3326-32-7
Fluorescein isothiocyanate isomer I
FLUORESCEIN-5-ISOTHIOCYANATE
fluorescein 5-isothiocyanate
Spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one, 3',6'-dihydroxy-5-isothiocyanato-
FITC-I
CHEBI:37918
EINECS 222-042-0
UNII-I223NX31W9
FITC, 5-
DTXSID80892440
Spiro(isobenzofuran-1(3H),9'-(9H)xanthen)-3-one, 3',6'-dihydroxy-5-isothiocyanato-
3',6'-dihydroxy-5-isothiocyanato-3H-spiro[2-benzofuran-1,9'-xanthen]-3-one
3',6'-dihydroxy-5-isothiocyanato-3H-spiro[2-benzofuran-1,9'-xanthene]-3-one
FITC-Celite(R)
5 Isothiocyanatofluorescein
UNII-7BUU93N0HM
Fluorescein 5 isothiocyanate
3',6'-dihydroxy-4-isothiocyanato-3H-spiro(2-benzofuran-1,9'-xanthen)-3-one
3',6'-dihydroxy-4-isothiocyanato-3H-spiro[2-benzofuran-1,9'-xanthen]-3-one
3',6'-dihydroxy-5-isothiocyanato-3H-spiro(2-benzofuran-1,9'-xanthen)-3-one
3',6'-dihydroxy-5-isothiocyanato-3H-spiro(2-benzofuran-1,9'-xanthene)-3-one
Fluorescein-5(or 6)-isothiocyanate
EINECS 248-207-7
Fluoreszeinisothiocyanat
Fluorescein isothiocyanate isomer I-Celite(R)
7BUU93N0HM
Fluorescein isothiocyanic acid
Fluorescein 5(6)isothiocyanate
DTXCID3029368
CHEBI:37926
Fluorescein5(or 6)isothiocyanate
FLUORESCEIN ISOTHIOCYANATE [WHO-DD]
3',6'dihydroxy5isothiocyanatospiro(isobenzofuran1(3H),9'(9H)xanthen)3one
3',6'Dihydroxy5(or 6)isothiocyanatospiro(isobenzofuran1(3H),9'(9H)xanthene)3one
Benzoic acid, 2-(6-hydroxy-3-oxo-3H-xanthen-9-yl)-, monoisothiocyanato deriv.
Spiro(isobenzofuran1(3H),9'(9H)xanthen)3one, 3',6'dihydroxy5(or 6)isothiocyanato
Spiro(isobenzofuran1(3H),9'(9H)xanthen)3one, 3',6'dihydroxy5isothiocyanato
222-042-0
248-207-7
25168-13-2
27072-45-3
fluorescein isothiocyanate
mhmnjmpurvtyej-uhfffaoysa-n
FITC
5-FITC
5-isothiocyanatofluorescein
Fluorescein 5(6)-isothiocyanate
3',6'-dihydroxy-5-isothiocyanato-3H-spiro[isobenzofuran-1,9'-xanthen]-3-one
FITC isomer 1
MFCD00005063
F5ITC
Fluorescein-5-isothiocyanat
Fluoreszein-5-isothiocyanat
2-(6-Hydroxy-3-oxo-(3H)-xanthen-9-yl)-5-isothiocyanatobenzoic acid
3',6'-dihydroxy-6-isothiocyanatospiro[2-benzofuran-3,9'-xanthene]-1-one
Fluorescein isothiocyanate isomer I, tech grade
I223NX31W9
fluorescein isothiocyanate isomer 1
Fluorescein 5-Isothiocyanate, Isomer 1, 95%
FITC ISOMER I
3',6'-dihydroxy-5-isothiocyanatospiro(isobenzofuran-1(3H),9'-(9H)xanthen)-3-one
FITC ISOMER
Fluorescein 5-Isothiocyanate, Isomer 1 (~90%)
Fluorescein isothiocyanate isomer I, >=97.5% (HPLC)
5-FITC(isomer I)
FITC?
FLUORESCEIN ISOTHIOCYANATE, ISOMER 1
Epitope ID:115575
DSSTox_CID_29368
DSSTox_RID_83483
Fluorescein-4-Isothiocyanate
DSSTox_GSID_49408
SCHEMBL71426
Fluorescein 5-isothiocyanate;
CHEMBL516313
5(6)-Fluorescein isothiocyanate
FITC, Fluorescein isothiocyanate
Fluorescein-5-isothiocyanate tech.
GLXC-10848
BCP04195
EX-A1778
Tox21_202818
5-FITC;5-Isothiocyanato fluorescein
Fluorescein-5-isothiocyanate (FITC)
s6928
AKOS015897625
FITC;5(6)-Fluorescein isothiocyanate
FF05796
FF35415
GS-6079
NCGC00260364-01
AC-37042
AC-37589
BP-23367
BP-30030
DA-53249
Fluorescein isothiocyanate isomer I, 90%
HY-66019
SY011095
CAS-27072-45-3
F0026
NS00028252
A818907
A821696
Fluorescein 5(6)-isothiocyanate, >=90% (HPLC)
F04-0012
Q59647575
Fluorescein-5-isothiocyanate; 5-Isothiocyanatofluorescein
3',6'-dihydroxy-6-isothiocyanato-1-spiro[isobenzofuran-3,9'-xanthene]one
3',6'-dihydroxy-6-isothiocyanato-spiro[isobenzofuran-3,9'-xanthene]-1-one
3',6'-Dihydroxy-5-isothiocyanato-spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one
6-isothiocyanato-3',6'-bis(oxidanyl)spiro[2-benzofuran-3,9'-xanthene]-1-one
Fluorescein isothiocyanate isomer I, suitable for protein labeling, >=90% (HPLC), powder
Fluorescein isothiocyanate isomer I-Celite(R), suitable for fluorescent labeling techniques