Disodium EDTA
- Molecular formula
- C10H14N2Na2O8
- CAS Number
- 139-33-3
- IUPAC Name
- sodium;2-[2-[2-[bis(carboxymethyl)amino]ethyl-(carboxymethyl)amino]ethyl-(carboxymethyl)amino]acetate
- Molecular Weight
- 372.24g/mol
- Melting Point
- (Celsius)
- Boiling Point
- (Celsius)
- Density
- 0.8602g/cm3
Identification
Interesting Facts about Sodium; 2-[2-[2-[bis(carboxymethyl)amino]ethyl-(carboxymethyl)amino]ethyl-(carboxymethyl)amino]acetate
Sodium; 2-[2-[2-[bis(carboxymethyl)amino]ethyl-(carboxymethyl)amino]ethyl-(carboxymethyl)amino]acetate, commonly known as sodium tris(carboxymethyl)aminomethane or simply "Tris", is an intriguing compound with a range of applications in biochemistry and molecular biology.
Key Highlights:
- Biological Buffer: Tris is widely used as a buffering agent in biological and biochemical research. It helps maintain a stable pH in a range of biochemical reactions.
- Importance in Electrophoresis: This compound is crucial in techniques such as SDS-PAGE and agarose gel electrophoresis, where pH stabilization is necessary for separating biomolecules like proteins and nucleic acids.
- Versatile pH Range: Tris is effective in a pH range of approximately 7 to 9, allowing researchers to customize experimental conditions according to their specific needs.
- Safety: It is generally considered safe for use, making it a preferred choice in many laboratory protocols.
Moreover, the structural features of Tris, characterized by multiple carboxymethyl groups, contribute to its effectiveness as a chelator, allowing it to interact meaningfully with ions and stabilize them in solution. This is particularly useful in enzymatic reactions that rely on metal ions.
As a chemist or student in molecular biology, understanding the role of Tris in research can open doors to innovative experiments and foster a deeper appreciation for the delicate balance of biological systems. As noted by one notable scientist, "The path to discovery often lies in the seemingly simple tools we use every day in the lab."
Overall, sodium; 2-[2-[2-[bis(carboxymethyl)amino]ethyl-(carboxymethyl)amino]ethyl-(carboxymethyl)amino]acetate is more than just a compound; it is an indispensable ally in the quest for knowledge in the life sciences.